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Those cells already contain genetic material, chromosomes full of genes, genes full of DNA. As with humans, chicken chromosomes determine sex. If a chicken receives two Z chromosomes, it will be male. If it receives a Z and a W chromosome, it will be female. That gives CSIRO’s genetic engineers a target: the female Z chromosome that’s passed down to male chicks.
The technology at the center of the breakthrough is CRISPR, a powerful tool discovered in 2012 that is so versatile it can edit almost any gene in almost any species. Often called a «pair of molecular scissors,» CRISPR can make a cut in the DNA sequence of a gene and «paste» a new gene in the gap.
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What happens if the egg doesn’t glow red? Then the chicken is female, the egg is placed in an incubator and 21 days later a hen is born. She is then sold to a farm and throughout her life she will pump out the eggs that fill cartons on supermarket shelves.
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In the case of CSIRO’s genetically engineered chickens, CRISPR would paste a gene derived from a sea anemone into the male-only chromosome. That gene produces «red fluorescent protein» (RFP), which glows under laser light, causing the cells to give off a red-orange fluorescence. Just like a red traffic light tells you to stop, seeing the red light reflecting from within the egg tells a handler (or machine) there’s a male chick inside, and the incubation process is terminated.
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Some of the costs may be partially offset for egg producers as new revenue streams are created with the excess male chicken eggs. Eggs are an important part of vaccine production, with up to half a billion eggs per year used to produce around 90 percent of the world’s flu vaccines. Egg whites are also prominent in the cosmetic industry due to their high protein content, where they can be used in shampoos and hair treatments.
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